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CAPRIN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CAPRIN1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G115657-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human GPIP137(CAPRIN1) (NM_005898), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Caprin 1 | CAPRIN1 | Caprin-1 | GPI-p137 | GPIP137 | M11S1 | GPIAP1 | p137GPI | RNG105 | GPI-anchored protein p137 | RNA granule protein 105
Origin Species
Human
NCBI GenBank
NM_005898
SwissProt
Q14444

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPSATSHSGS GSKSSGPPPP SGSSGSEAAA GAGAAAPASQ HPATGTGAVQ TEAMKQILGV
61IDKKLRNLEK KKGKLDDYQE RMNKGERLNQ DQLDAVSKYQ EVTNNLEFAK ELQRSFMALS
121QDIQKTIKKT ARREQLMREE AEQKRLKTVL ELQYVLDKLG DDEVRTDLKQ GLNGVPILSE
181EELSLLDEFY KLVDPERDMS LRLNEQYEHA SIHLWDLLEG KEKPVCGTTY KVLKEIVERV
241FQSNYFDSTH NHQNGLCEEE EAASAPAVED QVPEAEPEPA EEYTEQSEVE STEYVNRQFM
301AETQFTSGEK EQVDEWTVET VEVVNSLQQQ PQAASPSVPE PHSLTPVAQA DPLVRRQRVQ
361DLMAQMQGPY NFIQDSMLDF ENQTLDPAIV SAQPMNPTQN MDMPQLVCPP VHSESRLAQP
421NQVPVQPEAT QVPLVSSTSE GYTASQPLYQ PSHATEQRPQ KEPIDQIQAT ISLNTDQTTA
481SSSLPAASQP QVFQAGTSKP LHSSGINVNA APFQSMQTVF NMNAPVPPVN EPETLKQQNQ
541YQASYNQSFS SQPHQVEQTE LQQEQLQTVV GTYHGSPDQS HQVTGNHQQP PQQNTGFPRS
601NQPYYNSRGV SRGGSRGARG LMNGYRGPAN GFRGGYDGYR PSFSNTPNSG YTQSQFSAPR
661DYSGYQRDGY QQNFKRGSGQ SGPRGAPRGR GGPPRPNRGM PQMNTQQVNT RTRPLEQKLI
721SEEDLAANDI LDYKDDDDKV

740 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.