Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CAPN9 / Calpain 9 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G112632-100
Catalog Number: LS-G112632
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Calpain 9(CAPN9) (NM_016452), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Calpain 9 | CAPN9 | GC36 | NCL-4 | NCL4 | Protein CG36 | New calpain 4 | Calpain 9 (nCL-4) | Calpain-9 | Novel calpain large subunit-4
- Origin Species
- Human
- NCBI GenBank
- NM_006615
- SwissProt
- O14815
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 76 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPYLYRAPGP QAHPVPKDAR ITHSSGQSFE QMRQECLQRG TLFEDADFPA SNSSLFYSER61PQIPFVWKRP GEIVKNPEFI LGGATRTDIC QGELGDCWLL AAIASLTLNQ KALARVIPQD121QSFGPGYAGI FHFQFWQHSE WLDVVIDDRL PTFRDRLVFL HSADHNEFWS ALLEKAYAKL181NGSYEALKGG SAIEAMEDFT GGVAETFQTK EAPENFYEIL EKALKRGSLL GCFIDTRSAA241ESEARTPFGL IKGHAYSVTG IDQVSFRGQR IELIRIRNPW GQVEWNGSWS DRMAFKDFKA301HFDKVEICNL TPDALEEDAI HKWEVTVHQG SWVRGSTAGG CRNFLDTFWT NPQIKLSLTE361KDEGQEECSF LVALMQKDRR KLKRFGANVL TIGYAIYECP DKDEHLNKDF FRYHASRARS421KTFINLREVS DRFKLPPGEY ILIPSTFEPH QEADFCLRIF SEKKAITRDM DGNVDIDLPE481PPKPTPPDQE TEEEQRFRAL FEQVAGEDME VTAEELEYVL NAVLQKKKDI KFKKLSLISC541KNIISLMDTS GNGKLEFDEF KVFWDKLKQW INLFLRFDAD KSGTMSTYEL RTALKAAGFQ601LSSHLLQLIV LRYADEELQL DFDDFLNCLV RLENASRVFQ ALSTKNKEFI HLNINEFIHL661TMNITRTRPLEQKLISEEDLAANDILDYKDDDDKV
664 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.