Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CAPN8 / Calpain 8 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G126260-100
Catalog Number: LS-G126260
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CAPN8 (NM_001143962), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Calpain 8 | Calpain-8 | CAPN8 | Calpain 8 (nCL-2) | NCL-2 | NCL2 | New calpain 2
- Origin Species
- Human
- SwissProt
- A6NHC0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 79.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAAQAAGVSR QRAATQGLGS NQNALKYLGQ DFKTLRQQCL DSGVLFKDPE FPACPSALGY61KDLGPGSPQT QGIIWKRPTE LCPSPQFIVG GATRTDICQG GLGDCWLLAA IASLTLNEEL121LYRVVPRDQD FQENYAGIFH FQFWQYGEWV EVVIDDRLPT KNGQLLFLHS EQGNEFWSAL181LEKAYAKLNG CYEALAGGST VEGFEDFTGG ISEFYDLKKP PANLYQIIRK ALCAGSLLGC241SIDVSSAAEA EAITSQKLVK SHAYSVTGVE EVNFQGHPEK LIRLRNPWGE VEWSGAWSDD301APEWNHIDPR RKEELDKKVE DGEFWMSLSD FVRQFSRLEI CNLSPDSLSS EEVHKWNLVL361FNGHWTRGST AGGCQNYPAT YWTNPQFKIR LDEVDEDQEE SIGEPCCTVL LGLMQKNRRW421RKRIGQGMLS IGYAVYQVPK ELESHTDAHL GRDFFLAYQP SARTSTYVNL REVSGRARLP481PGEYLVVPST FEPFKDGEFC LRVFSEKKAQ ALEIGDVVAG NPYEPHPSEV DQEDDQFRRL541FEKLAGKDSE ITANALKILL NEAFSKRTDI KFDGFNINTC REMISLLDSN GTGTLGAVEF601KTLWLKIQKY LEIYWETDYN HSGTIDAHEM RTALRKAGFT LNSQVQQTIA LRYACSKLGI661NFDSFVACMI RLETLFKLFS LLDEDKDGMV QLSLAEWLCC VLVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
703 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.