Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CAPN7 / Calpain 7 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101189-100
Catalog Number: LS-G101189
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Calpain 7(CAPN7) (NM_014296), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Calpain 7 | Calpain like protease | CALPAIN7 | PALBH | Calpain-7 | CAPN7 | PalB homolog
- Origin Species
- Human
- NCBI GenBank
- NM_014296
- SwissProt
- Q9Y6W3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 92.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDATALERDA VQFARLAVQR DHEGRYSEAV FYYKEAAQAL IYAEMAGSSL ENIQEKITEY61LERVQALHSA VQSKSADPLK SKHQLDLERA HFLVTQAFDE DEKENVEDAI ELYTEAVDLC121LKTSYETADK VLQNKLKQLA RQALDRAEAL GEPLTKPVGK ISSTSVKPKP PPERAHFPLG181ANPFLERPQS FISPQSCDAQ GQRYTAEEIE VLRTTSKING IEYVPFMNVD LRERFAYPMP241FCDRWGKLPL SPKQKTTFSK WVRPEDLTNN PTMIYTVSSF SIKQTIVSDC SFVASLAISA301AYERRFNKKL ITGIIYPQNK DGEPEYNPCG KYMVKLHLNG VPRKVIIDDQ LPVDHKGELL361CSYSNNKSEL WVSLIEKAYM KVMGGYDFPG SNSNIDLHAL TGWIPERIAM HSDSQTFSKD421NSFRMLYQRF HKGDVLITAS TGMMTEAEGE KWGLVPTHAY AVLDIREFKG LRFIQLKNPW481SHLRWKGRYS ENDVENWTPE LQKYLNFDPR TAQKIDNGIF WISWDDLCQY YDVIYLSWNP541GLFKESTCIH STWDAKQGPV KDAYSLANNP QYKLEVQCPQ GGAAVWVLLS RHITDKDDFA601NNREFITMVV YKTDGKKVYY PADPPPYIDG IRINSPHYLT KIKLTTPGTH TFTLVVSQYE661KQNTIHYTVR VYSACSFTFS KIPSPYTLSK RINGKWSGQS AGGCGNFQET HKNNPIYQFH721IEKTGPLLIE LRGPRQYSVG FEVVTVSTLG DPGPHGFLRK SSGDYRCGFC YLELENIPSG781IFNIIPSTFL PKQEGPFFLD FNSIIPIKIT QLQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
813 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.