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CAPN14 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CAPN14 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CAPN14 antibodies →
$725.00each
Catalog Number: LS-G126537-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Calpain 14(CAPN14) (NM_001145122), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001145122

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
79.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSLWPPFRCR WKLAPRYSRR ASPQQPQQDF EALLAECLRN GCLFEDTSFP ATLSSIGSGS
61LLQKLPPRLQ WKRPPELHSN PQFYFAKAKR LDLCQGIVGD CWFLAALQAL ALHQDILSRV
121VPLNQSFTEK YAGIFRFWFW HYGNWVPVVI DDRLPVNEAG QLVFVSSTYK NLFWGALLEK
181AYAKLSGSYE DLQSGQVSEA LVDFTGGVTM TINLAEAHGN LWDILIEATY NRTLIGCQTH
241SGEKILENGL VEGHAYTLTG IRKVTCKHRP EYLVKLRNPW GKVEWKGDWS DSSSKWELLS
301PKEKILLLRK DNDGEFWMTL QDFKTHFVLL VICKLTPGLL SQEAAQKWTY TMREGRWEKR
361STAGGQRQLL QDTFWKNPQF LLSVWRPEEG RRSLRPCSVL VSLLQKPRHR CRKRKPLLAI
421GFYLYRMNKY HDDQRRLPPE FFQRNTPLSQ PDRFLKEKEV SQELCLEPGT YLIVPCILEA
481HQKSEFVLRV FSRKHIFYEI GSNSGVVFSK EIEDQNERQD EFFTKFFEKH PEINAVQLQN
541LLNQMTWSSL GSRQPFFSLE ACQGILALLD LNASGTMSIQ EFRDLWKQLK LSQKVFHKQD
601RGSGYLNWEQ LHAAMREAGI MLSDDVCQLM LIRYGGPRLQ MDFVSFIHLM LRVENMEDVF
661QNLTQDGKGI YLQKPEWMMM ALYS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

684 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.