Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CAPN13 / Calpain 13 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G106993-100
Catalog Number: LS-G106993
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Calpain 13(CAPN13) (NM_144575), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Calpain 13 | CAPN13 | CANP 13 | Calpain-13
- Origin Species
- Human
- NCBI GenBank
- NM_144575
- SwissProt
- Q6MZZ7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 76.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAYYQEPSVE TSIIKFKDQD FTTLRDHCLS MGRTFKDETF PAADSSIGQK LLQEKRLSNV61IWKRPQDLPG GPPHFILDDI SRFDIQQGGA ADCWFLAALG SLTQNPQYRQ KILMVQSFSH121QYAGIFRFRF WQCGQWVEVV IDDRLPVQGD KCLFVRPRHQ NQEFWPCLLE KAYAKLLGSY181SDLHYGFLED ALVDLTGGVI TNIHLHSSPV DLVKAVKTAT KAGSLITCAT PSGPTDTAQA241MENGLVSLHA YTVTGAEQIQ YRRGWEEIIS LWNPWGWGET EWRGRWSDGS QEWEETCDPR301KSQLHKKRED GEFWMSCQDF QQKFIAMFIC SEIPITLDHG NTLHEGWSQI MFRKQVILGN361TAGGPRNDAQ FNFSVQEPME GTNVVVCVTV AVTPSNLKAE DAKFPLDFQV ILAGSQRFRE421KFPPVFFSSF RNTVQSSNNK FRRNFTMTYH LSPGNYVVVA QTRRKSAEFL LRIFLKMPDS481DRHLSSHFNL RMKGSPSEHG SQQSIFNRYA QQRLDIDATQ LQGLLNQELL TGPPGDMFSL541DECRSLVALM ELKVNGRLDQ EEFARLWKRL VHYQHVFQKV QTSPGVLLSS DLWKAIENTD601FLRGIFISRE LLHLVTLRYS DSVGRVSFPS LVCFLMRLEA MAKTFRNLSK DGKGLYLTEM661EWMSLVMYNTRTRPLEQKLISEEDLAANDILDYKDDDDKV
669 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.