Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CAPN11 / Calpain 11 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G129109-100
Catalog Number: LS-G129109
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Calpain 11(CAPN11) (NM_007058), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Calpain11 | CANP 11 | CAPN11 | Calpain 11 | Calpain-11
- Origin Species
- Human
- NCBI GenBank
- NM_007058
- SwissProt
- Q9UMQ6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 84.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLYSPGPSLP ESAESLDGSQ EDKPRGSCAE PTFTDTGMVA HINNSRLKAK GVGQHDNAQN61FGNQSFEELR AACLRKGELF EDPLFPAEPS SLGFKDLGPN SKNVQNISWQ RPKDIINNPL121FIMDGISPTD ICQGILGDCW LLAAIGSLTT CPKLLYRVVP RGQSFKKNYA GIFHFQIWQF181GQWVNVVVDD RLPTKNDKLV FVHSTERSEF WSALLEKAYA KLSGSYEALS GGSTMEGLED241FTGGVAQSFQ LQRPPQNLLR LLRKAVERSS LMGCSIEVTS DSELESMTDK MLVRGHAYSV301TGLQDVHYRG KMETLIRVRN PWGRIEWNGA WSDSAREWEE VASDIQMQLL HKTEDGEFWM361SYQDFLNNFT LLEICNLTPD TLSGDYKSYW HTTFYEGSWR RGSSAGGCRN HPGTFWTNPQ421FKISLPEGDD PEDDAEGNVV VCTCLVALMQ KNWRHARQQG AQLQTIGFVL YAVPKEFQNI481QDVHLKKEFF TKYQDHGFSE IFTNSREVSS QLRLPPGEYI IIPSTFEPHR DADFLLRVFT541EKHSESWELD EVNYAEQLQE EKVSEDDMDQ DFLHLFKIVA GEGKEIGVYE LQRLLNRMAI601KFKSFKTKGF GLDACRCMIN LMDKDGSGKL GLLEFKILWK KLKKWMDIFR ECDQDHSGTL661NSYEMRLVIE KAGIKLNNKV MQVLVARYAD DDLIIDFDSF ISCFLRLKTM FTFFLTMDPK721NTGHICLSLE QWLQMTMWGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
739 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.