Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CAMK2G / CaMK II Gamma Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G105510-100
Catalog Number: LS-G105510
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CAMK2G (NM_172169), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CAMK2G | CamKII gamma | CaM kinase II subunit gamma | CaMK | CaMK II gamma | CAMK-II | CAMKG | Gamma CaMK II | CaM kinase II gamma | CaMK-II subunit gamma
- Origin Species
- Human
- NCBI GenBank
- L07044
- SwissProt
- Q13555
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MATTATCTRF TDDYQLFEEL GKGAFSVVRR CVKKTPTQEY AAKIINTKKL SARDHQKLER61EARICRLLKH PNIVRLHDSI SEEGFHYLVF DLVTGGELFE DIVAREYYSE ADASHCIHQI121LESVNHIHQH DIVHRDLKPE NLLLASKCKG AAVKLADFGL AIEVQGEQQA WFGFAGTPGY181LSPEVLRKDP YGKPVDIWAC GVILYILLVG YPPFWDEDQH KLYQQIKAGA YDFPSPEWDT241VTPEAKNLIN QMLTINPAKR ITADQALKHP WVCQRSTVAS MMHRQETVEC LRKFNARRKL301KGAILTTMLV SRNFSVGRQS SAPASPAASA AGLAGQAAKS LLNKKSDGGV KKRKSSSSVH361LMEPQTTVVH NATDGIKGST ESCNTTTEDE DLKVRKQEII KITEQLIEAI NNGDFEAYTK421ICDPGLTSFE PEALGNLVEG MDFHKFYFEN LLSKNSKPIH TTILNPHVHV IGEDAACIAY481IRLTQYIDGQ GRPRTSQSEE TRVWHRRDGK WLNVHYHCSG APAAPLQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
527 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.