Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CAMK2D / CaMKII Delta Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G127469-100
Catalog Number: LS-G127469
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CAMK2D (NM_172129), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CaM kinase II delta | CaM kinase II delta subunit | CaM kinase II subunit delta | CaM-kinase II delta chain | CaMK-II delta subunit | CAMK2D | CaMKIIdelta | CaMK II delta | CAMKD | CaMK-II subunit delta | CaMKII delta | Delta CaMK-II
- Origin Species
- Human
- NCBI GenBank
- NM_001221
- SwissProt
- Q13557
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASTTTCTRF TDEYQLFEEL GKGAFSVVRR CMKIPTGQEY AAKIINTKKL SARDHQKLER61EARICRLLKH PNIVRLHDSI SEEGFHYLVF DLVTGGELFE DIVAREYYSE ADASHCIQQI121LESVNHCHLN GIVHRDLKPE NLLLASKSKG AAVKLADFGL AIEVQGDQQA WFGFAGTPGY181LSPEVLRKDP YGKPVDMWAC GVILYILLVG YPPFWDEDQH RLYQQIKAGA YDFPSPEWDT241VTPEAKDLIN KMLTINPAKR ITASEALKHP WICQRSTVAS MMHRQETVDC LKKFNARRKL301KGAILTTMLA TRNFSAAKSL LKKPDGVKKR KSSSSVQMME STESSNTTIE DEDVKARKQE361IIKVTEQLIE AINNGDFEAY TKICDPGLTA FEPEALGNLV EGMDFHRFYF ENALSKSNKP421IHTIILNPHV HLVGDDAACI AYIRLTQYMD GSGMPKTMQS EETRVWHRRD GKWQNVHFHR481SGSPTVPIKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
489 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.