Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CAMK2B / CaMKII Beta Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G105553-100
Catalog Number: LS-G105553
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CAMK2B (NM_172079), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Beta-subunit of CaMK II | Cam kinase II beta | CaMK IIbeta | CaM-kinase II beta chain | CAMKB | Beta CaMK II | CaM kinase II beta subunit | CaM kinase II subunit beta | CaM kinase IIbeta | CAM2 | Camk ii beta | CAMK2 | CAMK2B | CaMKII beta | CaMK-II subunit beta | CaMKIIbeta
- Origin Species
- Human
- NCBI GenBank
- NM_001220
- SwissProt
- Q13554
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 57.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MATTVTCTRF TDEYQLYEDI GKGAFSVVRR CVKLCTGHEY AAKIINTKKL SARDHQKLER61EARICRLLKH SNIVRLHDSI SEEGFHYLVF DLVTGGELFE DIVAREYYSE ADASHCIQQI121LEAVLHCHQM GVVHRDLKPE NLLLASKCKG AAVKLADFGL AIEVQGDQQA WFGFAGTPGY181LSPEVLRKEA YGKPVDIWAC GVILYILLVG YPPFWDEDQH KLYQQIKAGA YDFPSPEWDT241VTPEAKNLIN QMLTINPAKR ITAHEALKHP WVCQRSTVAS MMHRQETVEC LKKFNARRKL301KGAILTTMLA TRNFSAAKSL LNKKADGVKP QTNSTKNSAA ATSPKGTLPP AALEPQTTVI361HNPVDGIKES SDSANTTIED EDAKARKQEI IKTTEQLIEA VNNGDFEAYA KICDPGLTSF421EPEALGNLVE GMDFHRFYFE NLLAKNSKPI HTTILNPHVH VIGEDAACIA YIRLTQYIDG481QGRPRTSQSE ETRVWHRRDG KWQNVHFHCS GAPVAPLQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
518 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.