Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CAMK1G / CaMKI gamma Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101674-100
Catalog Number: LS-G101674
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CaMKI gamma(CAMK1G) (NM_020439), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CaM kinase IG | CaMK-like CREB kinase III | CaM kinase I gamma | CaM-KI gamma | CLICK III | CLICK3 | CaMK I gamma | Gamma CaMK I | VWS1 | CAMK1G | CaMKI gamma | CaMKIG | CLICKIII | DJ272L16.1
- Origin Species
- Human
- NCBI GenBank
- NM_020439
- SwissProt
- Q96NX5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGRKEEDDCS SWKKQTTNIR KTFIFMEVLG SGAFSEVFLV KQRLTGKLFA LKCIKKSPAF61RDSSLENEIA VLKKIKHENI VTLEDIYEST THYYLVMQLV SGGELFDRIL ERGVYTEKDA121SLVIQQVLSA VKYLHENGIV HRDLKPENLL YLTPEENSKI MITDFGLSKM EQNGIMSTAC181GTPGYVAPEV LAQKPYSKAV DCWSIGVITY ILLCGYPPFY EETESKLFEK IKEGYYEFES241PFWDDISESA KDFICHLLEK DPNERYTCEK ALSHPWIDGN TALHRDIYPS VSLQIQKNFA301KSKWRQAFNA AAVVHHMRKL HMNLHSPGVR PEVENRPPET QASETSRPSS PEITITEAPV361LDHSVALPAL TQLPCQHGRR PTAPGGRSLN CLVNGSLHIS SSLVPMHQGS LAAGPCGCCS421SCLNIGSKGK SSYCSEPTLL KKANKKQNFK SEVMVPVKAS GSSHCRAGQT GVCLIMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
476 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.