Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CALCR / Calcitonin Receptor Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G128198-100
Catalog Number: LS-G128198
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Calcitonin receptor(CALCR) (NM_001164737), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Calcitonin receptor | CALCR | CRT | CT-R | CTR | CT receptor
- Origin Species
- Human
- NCBI GenBank
- NM_001742
- SwissProt
- P30988
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQFSGEKISG QRDLQKSKMR FTFTSRCLAL FLLLNHPTPI LPAFSNQTYP TIEPKPFLYV61VGRKKMMDAQ YKCYDRMQQL PAYQGEGPYC NRTWDGWLCW DDTPAGVLSY QFCPDYFPDF121DPSEKVTKYC DEKGVWFKHP ENNRTWSNYT MCNAFTPEKL KNAYVLYYLA IVGHSLSIFT181LVISLGIFVF FRKLTTIFPL NWKYRKALSL GCQRVTLHKN MFLTYILNSM IIIIHLVEVV241PNGELVRRDP VSCKILHFFH QYMMACNYFW MLCEGIYLHT LIVVAVFTEK QRLRWYYLLG301WGFPLVPTTI HAITRAVYFN DNCWLSVETH LLYIIHGPVM AALVVNFFFL LNIVRVLVTK361MRETHEAESH MYLKAVKATM ILVPLLGIQF VVFPWRPSNK MLGKIYDYVM HSLIHFQGFF421VATIYCFCNN EVQTTVKRQW AQFKIQWNQR WGRRPSNRSA RAAAAAAEAG DIPIYICHQE481LRNEPANNQG EESAEIIPLN IIEQESSATRTRPLEQKLISEEDLAANDILDYKDDDDKV
508 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.