Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CALCOCO1 / CoCoa Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G126153-100
Catalog Number: LS-G126153
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CoCoA(CALCOCO1) (NM_001143682), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CALCOCO1 | Calphoglin | PP13275 | Sarcoma antigen NY-SAR-3 | Cocoa | KIAA1536
- Origin Species
- Human
- NCBI GenBank
- NM_020898
- SwissProt
- Q9P1Z2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 67.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEESPLSRAP SRGGVNFLNV ARTYIPNTKV ECHYTLPPGT MPSASDWIGI FKVEAACVRD61YHTFVWSSVP ESTTDGSPIH TSVQFQEPRP MDELVTLEEA DGGSDILLVV PKATVLQNQL121DESQQERNDL MQLKLQLEGQ VTELRSRVQE LERALATARQ EHTELMEQYK GISRSHGEIT181EERDILSRQQ GDHVARILEL EDDIQTISEK VLTKEVELDR LRDTVKALTR EQEKLLGQLK241EVQADKEQSE AELEPLKEQL RGAQELAASS QQKATLLGEE LASAAAARDR TIAELHRSRL301EVAEVNGRLA ELGLHLKEEK CQWSKERAGL LQSVEAEKDK ILKLSAEILR LEKAVQEERT361QNQVFKTELA REKDSSLVQL SESKRELTEL RSALRVLQKE KEQLQEEKQE LLEYMRKLEA421RLEKVADEKW NEDATTEDEE AAVGLSCPAA LTDSEDESPE DMRLPPYGLC ERGDPGSSPA481GPREASPLVV ISQPAPISPH LSGPAEDSSS DSEAEDEKSV LMAAVQSGGE EANLLLPELG541SAFYDMASGF TVGTLSETST GGPATPTWKE CPICKERFPA ESDKDALEDH MDGHFFFSTQ601DPFTFETRTRPLEQKLISEEDLAANDILDYKDDDDKV
606 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.