LSBio
CADPS Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CADPS Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CADPS antibodies →
$725.00each
Catalog Number: LS-G104030-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CAPS1(CADPS) (NM_183393), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CADPS | CAPS-1 | CAPS1 | CADPS1 | KIAA1121
Origin Species
Human
NCBI GenBank
NM_003716
SwissProt
Q9ULU8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
143.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLDPSSSEEE SDEIVEEESG KEVLGSAPSG ARLSPSRTSE GSAGSAGLGG GGAGAGAGVG
61AGGGGGSGAS SGGGAGGLQP SSRAGGGRPS SPSPSVVSEK EKEELERLQK EEEERKKRLQ
121LYVFVMRCIA YPFNAKQPTD MARRQQKISK QQLQTVKDRF QAFLNGETQI MADEAFMNAV
181QSYYEVFLKS DRVARMVQSG GCSANDSREV FKKHIEKRVR SLPEIDGLSK ETVLSSWMAK
241FDAIYRGEED PRKQQARMTA SAASELILSK EQLYEMFQNI LGIKKFEHQL LYNACQLDNP
301DEQAAQIRRE LDGRLQMADQ IARERKFPKF VSKEMENMYI EELKSSVNLL MANLESMPVS
361KGGEFKLQKL KRSHNASIID MGEESENQLS KSDVVLSFSL EVVIMEVQGL KSLAPNRIVY
421CTMEVEGGEK LQTDQAEASK PTWGTQGDFS TTHALPAVKV KLFTESTGVL ALEDKELGRV
481ILHPTPNSPK QSEWHKMTVS KNCPDQDLKI KLAVRMDKPQ NMKHSGYLWA IGKNVWKRWK
541KRFFVLVQVS QYTFAMCSYR EKKAEPQELL QLDGYTVDYT DPQPGLEGGR AFFNAVKEGD
601TVIFASDDEQ DRILWVQAMY RATGQSHKPV PPTQVQKLNA KGGNVPQLDA PISQFYADRA
661QKHGMDEFIS SNPCNFDHAS LFEMGWFSPG QVFVLDEYCA RNGVRGCHRH LCYLRDLLER
721AENGAMIDPT LLHYSFAFCA SHVHGNRPDG IGTVTVEEKE RFEEIKERLR VLLENQITHF
781RYCFPFGRPE GALKATLSLL ERVLMKDIVT PVPQEEVKTV IRKCLEQAAL VNYSRLSEYA
841KIEENVGRLI TPAKKLEDTI RLAELVIEVL QQNEEHHAEA FAWWSDLMVE HAETFLSLFA
901VDMDAALEVQ PPDTWDSFPL FQLLNDFLRT DYNLCNGKFH KHLQDLFAPL VVRYVDLMES
961SIAQSIHRGF ERESWEPVNN GSGTSEDLFW KLDALQTFIR DLHWPEEEFG KHLEQRLKLM
1021ASDMIESCVK RTRIAFEVKL QKTSRSTDFR VPQSICTMFN VMVDAKAQST KLCSMEMGQE
1081HQYHSKIDEL IEETVKEMIT LLVAKFVTIL EGVLAKLSRY DEGTLFSSFL SFTVKAASKY
1141VDVPKPGMDV ADAYVTFVRH SQDVLRDKVN EEMYIERLFD QWYNSSMNVI CTWLTDRMDL
1201QLHIYQLKTL IRMVKKTYRD FRLQGVLDST LNSKTYETIR NRLTVEEATA SVSEGGGLQG
1261ISMKDSDEED EEDD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1274 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.