Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CACUL1 / Cullin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G105688-100
Catalog Number: LS-G105688
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C10orf46(CACUL1) (NM_153810), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cdk-associated cullin1 | CAC1 | C10orf46 | CACUL1 | Cullin
- Origin Species
- Human
- NCBI GenBank
- AA442432
- SwissProt
- Q86Y37
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 40.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MEESMEEEEG GSYEAMMDDQ NHNNWEAAVD GFRQPLPPPP PPSSIPAPAR EPPGGQLLAV61PAVSVDRKGP KEGLPMGPQP PPEANGVIMM LKSCDAAAAV AKAAPAPTAS STININTSTS121KFLMNVITIE DYKSTYWPKL DGAIDQLLTQ SPGDYIPISY EQIYSCVYKC VCQQHSEQMY181SDLIKKITNH LERVSKELQA SPPDLYIERF NIALGQYMGA LQSIVPLFIY MNKFYIETKL241NRDLKDDLIK LFTEHVAEKH IYSLMPLLLE AQSTPFQVTP STMANIVKGL YTLRPEWVQM301APTLFSKFIP NILPPAVESE LSEYAAQDQK FQRELIQNGF TRGDQSRKRA GDELAYNSSS361ACASSRGYRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
369 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.