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CACNA2D1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CACNA2D1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CACNA2D1 antibodies →
$725.00each
Catalog Number: LS-G123022-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CACNA2D1 (NM_000722), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Alpha2delta-1 | CCHL2A | CACNA2D1 | CACNA2 | CACNL2A | DHSCCA | MHS3 | RB-alpha 2 | DHPR delta
Origin Species
Human
NCBI GenBank
NM_000722
SwissProt
P54289

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
123 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAGCLLALT LTLFQSLLIG PSSEEPFPSA VTIKSWVDKM QEDLVTLAKT ASGVNQLVDI
61YEKYQDLYTV EPNNARQLVE IAARDIEKLL SNRSKALVRL ALEAEKVQAA HQWREDFASN
121EVVYYNAKDD LDPEKNDSEP GSQRIKPVFI EDANFGRQIS YQHAAVHIPT DIYEGSTIVL
181NELNWTSALD EVFKKNREED PSLLWQVFGS ATGLARYYPA SPWVDNSRTP NKIDLYDVRR
241RPWYIQGAAS PKDMLILVDV SGSVSGLTLK LIRTSVSEML ETLSDDDFVN VASFNSNAQD
301VSCFQHLVQA NVRNKKVLKD AVNNITAKGI TDYKKGFSFA FEQLLNYNVS RANCNKIIML
361FTDGGEERAQ EIFNKYNKDK KVRVFTFSVG QHNYDRGPIQ WMACENKGYY YEIPSIGAIR
421INTQEYLDVL GRPMVLAGDK AKQVQWTNVY LDALELGLVI TGTLPVFNIT GQFENKTNLK
481NQLILGVMGV DVSLEDIKRL TPRFTLCPNG YYFAIDPNGY VLLHPNLQPK NPKSQEPVTL
541DFLDAELEND IKVEIRNKMI DGESGEKTFR TLVKSQDERY IDKGNRTYTW TPVNGTDYSL
601ALVLPTYSFY YIKAKLEETI TQARSKKGKM KDSETLKPDN FEESGYTFIA PRDYCNDLKI
661SDNNTEFLLN FNEFIDRKTP NNPSCNADLI NRVLLDAGFT NELVQNYWSK QKNIKGVKAR
721FVVTDGGITR VYPKEAGENW QENPETYEDS FYKRSLDNDN YVFTAPYFNK SGPGAYESGI
781MVSKAVEIYI QGKLLKPAVV GIKIDVNSWI ENFTKTSIRD PCAGPVCDCK RNSDVMDCVI
841LDDGGFLLMA NHDDYTNQIG RFFGEIDPSL MRHLVNISVY AFNKSYDYQS VCEPGAAPKQ
901GAGHRSAYVP SVADILQIGW WATAAAWSIL QQFLLSLTFP RLLEAVEMED DDFTASLSKQ
961SCITEQTQYF FDNDSKSFSG VLDCGNCSRI FHGEKLMNTN LIFIMVESKG TCPCDTRLLI
1021QAEQTSDGPN PCDMVKQPRY RKGPDVCFDN NVLEDYTDCG GVSGLNPSLW YIIGIQFLLL
1081WLVSGSTHRL L
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1091 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.