Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CACNA1S / Cav1.1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G123414-100
Catalog Number: LS-G123414
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CACNA1S (NM_000069), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Alpha 1S | Cav1.1 | CACNL1A3 | CACN1 | CCHL1A3 | DHPR alpha 1S | Dihydropyridine receptor | HOKPP1 | HypoPP | MHS5 | HOKPP | TTPP1 | CACH1 | CACNA1S
- Origin Species
- Human
- NCBI GenBank
- NM_000069
- SwissProt
- Q13698
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 212.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEPSSPQDEG LRKKQPKKPV PEILPRPPRA LFCLTLENPL RKACISIVEW KPFETIILLT61IFANCVALAV YLPMPEDDNN SLNLGLEKLE YFFLIVFSIE AAMKIIAYGF LFHQDAYLRS121GWNVLDFTIV FLGVFTVILE QVNVIQSHTA PMSSKGAGLD VKALRAFRVL RPLRLVSGVP181SLQVVLNSIF KAMLPLFHIA LLVLFMVIIY AIIGLELFKG KMHKTCYFIG TDIVATVENE241EPSPCARTGS GRRCTINGSE CRGGCPGPNH GITHFDNFGF SMLTVYQCIT MEGWTDVLYW301VNDAIGNEWP WIYFVTLILL GSFFILNLVL GVLSGEFTKE REKAKSRGTF QKLREKQQLD361EDLRGYMSWI TQGEVMDVED FREGKLSLDE GGSDTESLYE IAGLNKIIQF IRHWRQWNRI421FRWKCHDIVK SKVFYWLVIL IVALNTLSIA SEHHNQPHWL TRLQDIANRV LLSLFTTEML481MKMYGLGLRQ YFMSIFNRFD CFVVCSGILE ILLVESGAMT PLGISVLRCI RLLRIFKITK541YWTSLSNLVA SLLNSIRSIA SLLLLLFLFI VIFRLLGMQL FGGRYDFEDT EVRRSNFDNF601PQALISVFQV LTGEDWTSMM YNGIMASSGP SYPGMLVCIY FIILFVCGNY ILLNVFLAIA661VDNLAEAESL TSAQKAKAEE KKRRKMSKGL PDKSEEEKST MAKKLEQKPK GEGIPTTAKL721KIDEFESNVN EVKDPYPSAD FPGDDEEDEP EIPLSPRPRP LAELQLKEKA VPIPEASSFF781IFSPTNKIRV LCHRIVNATW FTNFILLFIL LSSAALAAED PIRADSMRNQ ILKHFDIGFT841SVFTVEIVLK MTTYGAFLHK GSFCRNYFNM LDLLVVAVSL ISMGLESSAI SVVKILRVLR901VLRPLRAINR AKGLKHVARC MFVAISTIGN IVLVTTLLQF MFACIGVQLF KGKFFRCTDL961SKMTEEECRG YYYVYKDGDP MQIELRHREW VHSDFHFDNV LSAMMSLFTV STFEGWPQLL1021YKAIDSNAED VGPIYNNRVE MAIFFIIYII LIAFFMMNIF VGFVIVTFQE QGETEYKNCE1081LDKNQRQCVQ YALKARPLRC YIPKNPYQYQ VWYIVTSSYF EYLMFALIML NTICLGMQHY1141NQSEQMNHIS DILNVAFTII FTLEMILKLM AFKARGYFGN PWNVFDFLIV IGSIIDVILS1201EIDTFLASSG GLYCLGGGCG NVDPDESARI SSAFFRLFRV MRLIKLLSRA EGVRTLLWTF1261IKSFQALPYV ALLIVMLFFI YAVIGMQMFG KIALVDGTQI NRNNNFQTFP QAVLLLFRCA1321TGEAWQEILL ACSYGKLCDP ESDYAPGEEY TCGTNFAYYY FISFYMLCAF LVINLFVAVI1381MDNFDYLTRD WSILGPHHLD EFKAIWAEYD PEAKGRIKHL DVVTLLRRIQ PPLGFGKFCP1441HRVACKRLVG MNMPLNSDGT VTFNATLFAL VRTALKIKTE GNFEQANEEL RAIIKKIWKR1501TSMKLLDQVI PPIGDDEVTV GKFYATFLIQ EHFRKFMKRQ EEYYGYRPKK DIVQIQAGLR1561TIEEEAAPEI CRTVSGDLAA EEELERAMVE AAMEEGIFRR TGGLFGQVDN FLERTNSLPP1621VMANQRPLQF AEIEMEEMES PVFLEDFPQD PRTNPLARAN TNNANANVAY ANSNHSNSHV1681FSSVHYEREF PEETETPATR GRALGQPCRS LGPHSKPCVE MLKGLLTQRA MPRGQAPPAP1741CQCPRVESSM PEDRKSSTPG SLHEETPHSR STRENTSRCS APATALLIQK ALVRGGLGTL1801AADANFIMAT GQALGDACQM EPEEVEIMAT ELLKGREAPD GMASSLGCLN LGSSLGSLDQ1861HQGSQETLIP PRLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1873 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.