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CACHD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CACHD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CACHD1 antibodies →
$725.00each
Catalog Number: LS-G110907-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CACHD1 (NM_020925), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CACHD1 | RP4-655E10.1 | Cache domain containing 1 | KIAA1573 | VWCD1
Origin Species
Human
NCBI GenBank
AB046793
SwissProt
Q5VU97

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
136.5 kda
Protein Type
Lysate
Amino Acid Sequence
1MRRLAAEELG VVTMQRIFNS FVYTEKISNG ESEVQQLAKK IREKFNRYLD VVNRNKQVVE
61ASYTAHLTSP LTAIQDCCTI PPSMMEFDGN FNTNVSRTIS CDRLSTTVNS RAFNPGRDLN
121SVLADNLKSN PGIKWQYFSS EEGIFTVFPA HKFRCKGSYE HRSRPIYVST VRPQSKHIVV
181ILDHGASVTD TQLQIAKDAA QVILSAIDEH DKISVLTVAD TVRTCSLDQC YKTFLSPATS
241ETKRKMSTFV SSVKSSDSPT QHAVGFQKAF QLIRSTNNNT KFQANTDMVI IYLSAGITSK
301DSSEEDKKAT LQVINEENSF LNNSVMILTY ALMNDGVTGL KELAFLRDLA EQNSGKYGVP
361DRMALPVIKG SMMVLNQLSN LETTVGRFYT NLPNRMIDEA VFSLPFSDEM GDGLIMTVSK
421PCYFGNLLLG IVGVDVNLAY ILEDVTYYQD SLASYTFLID DKGYTLMHPS LTRPYLLSEP
481PLHTDIIHYE NIPKFELVRQ NILSLPLGSQ IIAVPVNSSL SWHINKLRET GKEAYNVSYA
541WKMVQDTSFI LCIVVIQPEI PVKQLKNLNT VPSSKLLYHR LDLLGQPSAC LHFKQLATLE
601SPTIMLSAGS FSSPYEHLSQ PETKRMVEHY TAYLSDNTRL IANPGLKFSV RNEVMATSHV
661TDEWMTQMEM SSLNTYIVRR YIATPNGVLR IYPGSLMDKA FDPTRRQWYL HAVANPGLIS
721LTGPYLDVGG AGYVVTISHT IHSSSTQLSS GHTVAVMGID FTLRYFYKVL MDLLPVCNQD
781GGNKIRCFIM EDRGYLVAHP TLIDPKGHAP VEQQHITHKE PLVANDILNH PNFVKKNLCN
841SFSDRTVQRF YKFNTSLAGD LTNLVHGSHC SKYRLARIPG TNAFVGIVNE TCDSLAFCAC
901SMVDRLCLNC HRMEQNECEC PCECPLEVNE CTGNLTNAEN RNPSCEVHQE PVTYTAIDPG
961LQDALHQCVN SRCSQRLESG DCFGVLDCEW CMVDSDGKTH LDKPYCAPQK ECFGGIVGAK
1021SPYVDDMGAI GDEVITLNMI KSAPVGPVAG GIMGCIMVLV LAVYAYRHQI HRRSHQHMSP
1081LAAQEMSVRM SNLENDRDER DDDSHEDRGI ISNTRFIAAV IERHAHSPER RRRYWGRSGT
1141ESDHGYSTMS PQEDSENPPC NNDPLSAGVD VGNHDEDLDL DTPPQTAALL SHKFHHYRSH
1201HPTLHHSHHL QAAVTVHTVD AEC
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1223 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.