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CABYR Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

CABYR Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all CABYR antibodies →
$725.00each
Catalog Number: LS-G114582-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CABYR (NM_012189), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CABYRc/d | Calcium-binding protein 86 | Cancer/testis antigen 88 | CABYR | CABYRe | CBP86 | Fibrousheathin 2 | Fibrousheathin II | FSP-2 | FSP2 | CABYRa | CABYRc | CT88 | Fibrousheathin-2
Origin Species
Human
NCBI GenBank
NM_012189
SwissProt
O75952

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
52.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MISSKPRLVV PYGLKTLLEG ISRAVLKTNP SNINQFAAAY FQELTMYRGN TTMDIKDLVK
61QFHQIKVEKW SEGTTPQKKL ECLKEPGKTS VESKVPTQME KSTDTDEDNV TRTEYSDKTT
121QFPSVYAVPG TEQTEAVGGL SSKPATPKTT TPPSSPPPTA VSPEFAYVPA DPAQLAAQML
181GKVSSIHSDQ SDVLMVDVAT SMPVVIKEVP SSEAAEDVMV AAPLVCSGKV LEVQVVNQTS
241VHVDLGSQPK ENEAEPSTAS SVPLQDEQEP PAYDQAPEVT LQADIEVMST VHISSVYNDV
301PVTEGVVYIE QLPEQIVIPF TDQVACLKEN EQSKENEQSP RVSPKSVVEK TTSGMSKKSV
361ESVKLAQLEE NAKYSSVYME AEATALLSDT SLKGQPEVPA QLLDAEGAIK IGSEKSLHLE
421VEITSIVSDN TGQEESGENS VPQEMEGKPV LSGEAAEAVH SGTSVKSSSG PFPPAPEGLT
481APEIEPEGES TAE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

493 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.