Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
CA14 / Carbonic Anhydrase XIV Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114631-100
Catalog Number: LS-G114631
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Carbonic Anhydrase XIV(CA14) (NM_012113), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CA14 | Carbonate dehydratase XIV | Carbonic anhydrase 14 | Carbonic dehydratase | CAXiV | CA-XIV | Carbonic anhydrase XIV
- Origin Species
- Human
- NCBI GenBank
- NM_012113
- SwissProt
- Q9ULX7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 37.67 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLFSALLLEV IWILAADGGQ HWTYEGPHGQ DHWPASYPEC GNNAQSPIDI QTDSVTFDPD61LPALQPHGYD QPGTEPLDLH NNGHTVQLSL PSTLYLGGLP RKYVAAQLHL HWGQKGSPGG121SEHQINSEAT FAELHIVHYD SDSYDSLSEA AERPQGLAVL GILIEVGETK NIAYEHILSH181LHEVRHKDQK TSVPPFNLRE LLPKQLGQYF RYNGSLTTPP CYQSVLWTVF YRRSQISMEQ241LEKLQGTLFS TEEEPSKLLV QNYRALQPLN QRMVFASFIQ AGSSYTTGEM LSLGVGILVG301CLCLLLAVYF IARKIRKKRL ENRKSVVFTS AQATTEATRTRPLEQKLISEEDLAANDILDYKDDDDKV
337 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.