LSBio
C9orf84 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C9orf84 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all C9orf84 antibodies →
$725.00each
Catalog Number: LS-G105188-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C9orf84 (NM_173521), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001080551

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
165 kDa
Protein Type
Lysate
Amino Acid Sequence
1MFSALKYHAI DYLYENVVRK KFYRDALLLR IPSCLYQDES YHVAVTDNKF RRPWTRVSAV
61SVPGMTDTSV LDQWKASFFV EDFLEKKTIT RMVTQINCEF EEVVPSSNPD SQIEVEEVSL
121YTHMDYNEVF TPVSCLEKCS ALQNQNQDLF IDDKGILFVS SRKHLPTLPT LLSRLKLFLV
181KDPLLDFKGQ IFTEANFSRE CFSLQETLEA FVKEDFCMDK VNFCQEKLED TICLNEPSSF
241LIEYEFLIPP SLKPEIDIPS LSELKELLNP VPEIINYVDE KEKLFERDLT NKHGIEDIGD
301IKFSSTEILT IQSQSEPEEC SKPGELEMPL TPLFLTCQHS SVNSLRTELQ TFPLSPVCKI
361NLLTAEESAN EYYMMWQLER CRSPLNPFLL TVPRIQEPHS QYSVTDLKKI FSVKEESLVI
421NLEKAEWWKQ AGLNLKMMET LEHLNTYLCH DNLSSNDTKI EIFLPTKVLQ LESCLEHKSR
481SSPIALIDEK STNAHLSLPQ KSPSLAKEVP DLCFSDDYFS DKGAAKEEKP KNDQEPVNRI
541IQKKENNDHF ELDCTGPSIK SPSSSIIKKA SFEHGKKQEN DLDLLSDFIM LRNKYKTCTS
601KTEVTNSDEK HDKEACSLTL QEESPIVHIN KTLEEINQER GTDSVIEIQA SDSQCQAFCL
661LEAAASPILK NLVSLCTLPT ANWKFATVIF DQTRFLLKEQ EKVVSDAVRQ GTIDEREMTF
721KHAALLHLLV TIRDVLLTCS LDTALGYLSK AKDIYNSILG PYLGDIWRQL EIVQFIRGKK
781PETNYKIQEL QCQILSWMQS QQQIKVLIII RMDSDGEKHF LIKILNKIEG LTLTVLHSNE
841RKDFLESEGV LRGTSSCVVV HNQYIGADFP WSNFSFVVEY NYVEDSCWTK HCKELNIPYM
901AFKVILPDTV LERSTLLDRF GGFLLEIQIP YVFFASEGLL NTPDILQLLE SNYNISLVER
961GCSESLKLFG SSECYVVVTI DEHTAIILQD LEELNCEKAS DNIIMRLMAL SLQYRYCWII
1021LYTKETLNSE YPLTEKTLHH LALIYAALVS FGLNSEELDV KLIIAPGVEA TALIIRQIAD
1081HSLMTSKRDP HEWLDKSWLK VSPSEEEMYL LDFPCINPLV AQLMLNKGPS LHWILLATLC
1141QLQELLPEVP EKVLKHFCSI TSLFKIGSSS ITKSPQISSP QENRNQISTL SSQSSASDLD
1201SVIQEHNEYY QYLGLGETVQ EDKTTTLNDN SSIMELKGIS SFLPPVTSYN QTSYWKDSSC
1261KSNIGQNTPF LINIESRRPA YNSFLNHSDS ESDVFSLGLT QMNCETIKSP TDTQKRVSVV
1321PRFINSQKRR THEAKGFINK DVSDPIFSLE GTQSPLHWNF KKNIWEQENH PFNLQYGAQQ
1381TACNKLYSQK GNLFTDQQKC LSDESEGLTC ESSKDETFWR ELPSVPSLDL FRASDSNANQ
1441KEFNSLYFYQ RAGKSLGQKR HHESSFNSGD KESLTGFMCS QLPQFKKRRL AYEKVPGRVD
1501GQTRLRFF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1508 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.