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C9orf117 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C9orf117 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G121399-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C9orf117(C9orf117) (NM_001012502), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C9orf117 | RP11-56D16.5
Origin Species
Human
SwissProt
Q5JU67

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPKKSVSKA GKELEVKKKG GKKEPVVAVE PPLAKEMKEF YHIQIRDLED RLARYQRKWD
61ELAVQEKMFR QEFEQLANNK KEIVAFLKRT LNQQVDEITD LNEQLQNLQL AKEMEKDAFE
121AQLAQVRHEF QETKDQLTTE NIILGGKLAA LEEFRLQKEE VTDKFTLLEE QVRKQENEFR
181DYAYNLEKKS VLDKDRLRKE IIQRVNLVAN EFHKVTTNRM WETTKRAIKE NNGITLQMAR
241VSQQGMKLLQ ENEQLKGRQN NLCKQLELLE NTQKVMARHK RGHQKIILML TKKCQEQQQD
301TKEAEELRLL LSQLEQRSLQ LQVDNQALKS QRDQLSLQLE QQQVDLQRLQ QELANEQKVR
361ASLEAALVQA TSFLQNILQM HRDEEDSDVD VTFQPWHKEM LQQLLVMLSS TVATRPQKAA
421CPHQESQSHG PPKESRPSIQ LPRTGSLLPQ LSDITPYQPG DLGLVPRQVH IPPNPQDLRL
481LSYITRVGTF RAHSSPEMRA PGSLKRLEKF SLPEVPLRPK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

520 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.