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C7orf31 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C7orf31 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all C7orf31 antibodies →
$504.00each
Catalog Number: LS-G107206-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C7orf31 (NM_138811), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C7orf31
Origin Species
Human
SwissProt
Q8N865

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MEVIHGRPYC CRELEGADIL SNTFYSNELH NPLQTVTRPT ASEDRYQELR ESLQQCRLPW
61GAEREYGGII PISLPEDHRP KCEPPRVMGK GHQHYGFGGE TWPRKLPVEQ FYYLTQNKKS
121DVYGNDSLIP KPPNSTVGEI CLQYPIEHPY HTHICRGAMF PTFTSPEDLY TGIKARTQQP
181FPPTVPSKAY DSTVLKTRGN PYRYELIDIP MDSKKKALTW PGQGVYYDFP RGVEKNKPVF
241YPKPPKTFAP NTSLNSWDPI CSAKEANIQR NLERSHWLTS YTHDFTGLGP MDPLELDDYH
301EKMVAELTRK IGFDPEPQEK FHPVFKPPRP LEGRIARLIQ NRRSLEAIVQ QRPRSCPDCT
361PRVLCNFHTF VPSSKEMVAL SDNIPAGVTH KNQDIEEKII EEQSLLSTYE LPSCYPTKDL
421TSIYDIKPFP KITDTKKTED LYWRQQSLKT QPTPYCKPDH WIHYENLKSP LRDQYNMCPD
481PVSLSKPSVL QNKQDTEAFT LEHFLSKPEE ELFLNMEDNE ETRPVLGWIP RAGVTKPQTN
541LLELKNSFSK TGAQKRFHKS ILEDHKDLRD NEHSGMKHQF YGHNSYYFYN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

590 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.