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C7orf25 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C7orf25 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G118859-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C7orf25 (NM_001099858), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C7orf25 | UPF0415 protein C7orf25
Origin Species
Human
NCBI GenBank
NM_024054
SwissProt
Q9BPX7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
52.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MQEVPEAAGL ARVEPPRRSS GVGAARLRFP GGSRPLRARA CVLALAVLAL PERNNADSMS
61AHSMLCERIA IAKELIKRAE SLSRSRKGGI EGGAKLCSKL KAELKFLQKV EAGKVAIKES
121HLQSTNLTHL RAIVESAENL EEVVSVLHVF GYTDTLGEKQ TLVVDVVANG GHTWVKAIGR
181KAEALHNIWL GRGQYGDKSI IEQAEDFLQA SHQQPVQYSN PHIIFAFYNS VSSPMAEKLK
241EMGISVRGDI VAVNALLDHP EELQPSESES DDEGPELLQV TRVDRENILA SVAFPTEIKV
301DVCKRVNLDI TTLITYVSAL SYGGCHFIFK EKVLTEQAEQ ERKEQVLPQL EAFMKDKELF
361ACESAVKDFQ SILDTLGGPG ERERATVLIK RINVVPDQPS ERALRLVASS KINSRSLTIF
421GTGDTLKAIT MTANSGFVRA ANNQGVKFSV FIHQPRALTE SKEALATPLP KDYTTDSEH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

479 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.