Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
C6orf47 / G4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110710-100
Catalog Number: LS-G110710
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C6orf47 (NM_021184), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C6orf47 | D6S53E | G4 | NG34 | Protein G4
- Origin Species
- Human
- NCBI GenBank
- NM_021184
- SwissProt
- O95873
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 31.5 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MFLRRLGGWL PRPWGRRKPM RPDPPYPEPR RVDSSSENSG SDWDSAPETM EDVGHPKTKD61SGALRVSRAA SEPSKEEPQV EQLGSKRMDS LKWDQPISST QESGRLEAGG ASPKLRWDHV121DSGGTRRPGV SPEGGLSVPG PGAPLEKPGR REKLLGWLRG EPGAPSRYLG GPEECLQIST181NLTLHLLELL ASALLALCSR PLRAALDTLG LRGPLGLWLH GLLSFLAALH GLHAVLSLLT241AHPLHFACLF GLLQALVLAV SLREPNGDEA ATDWESEGLE REGEEQRGDP GKGLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
294 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.