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C6orf146 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C6orf146 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all FAM217A antibodies →
$504.00each
Catalog Number: LS-G105213-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C6orf146(FAM217A) (NM_173563), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C6orf146 | Protein FAM217A | FAM217A
Origin Species
Human
NCBI GenBank
AA934994
SwissProt
Q8IXS0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGRRNENCAN SLRVSNISQE NLSHWNLDSE VPVSENKNLP AGRDGAAGGK INKNYLEIPV
61EQLMLEPNLS VHSQKSTQNS KQGIFQLWNC PLNEGSTIEK REFKKSSVET GFNVINHPIR
121VFTLNHPLTI ASVDKQVGPY PGLPMPLGLC WPYADGDFFK NRNEIHVSSC STIENNDGET
181LPAPNWNLKH GNSSVEENFT DESDLSENEK TNDTLLSYFK KVDLNLKPET IKNVEEPFTE
241EPNEVFPYPD FLPPPFSALD LHNLALSKSD NWKVTVDPAE TSVEHLITRL LELERLQHMT
301IQKERPRLQT TFCTPAVTER PSSSKATPKV RQPKLCDSLS LQIPCVDKSQ EKSKNNSGSC
361KLEQNALKRN WSNAGKYRWN SRPLSLKSSS TPKQLIETYD KNPKSSILSP CQELSFKPTI
421GHTNQSMVKM ISTRCLPWRS PMPVSPIPLT FPENQKEEIK APKRNFGTKK KLYRQNIVLN
481RPFSIQKLNC LSPSLIAKDK CCSPIEQK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

508 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.