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C5orf51 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C5orf51 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G104951-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C5orf51 (NM_175921), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C5orf51 | UPF0600 protein C5orf51 | LOC285636
Origin Species
Human
NCBI GenBank
NM_175921
SwissProt
A6NDU8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
33.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAAVSSVVR RVEELGDLAQ AHIQQLSEAA GEDDHFLIRA SAALEKLKLL CGEEKECSNP
61SNLLELYTQA ILDMTYFEEN KLVDEDFPED SSSQKVKELI SFLSEPEILV KENNMHPKHC
121NLLGDELLEC LSWRRGALLY MYCHSLTKRR EWLLRKSSLL KKYLLDGISY LLQMLNYRCP
181IQLNEGVSFQ DLDTAKLLSA GIFSDIHLLA MMYSGEMCYW GSKYCADQQP ENHEVDTSVS
241GAGCTTYKEP LDFREVGEKI LKKYVSVCEG PLKEQEWNTT NAKQILNFFH HRCN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

294 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.