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C5orf34 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C5orf34 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G103627-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C5orf34 (NM_198566), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_198566

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAELRMILY EDDSVQVQYV DGSTLQLSPC GSEFLFEKSP PVSAHPLEQP ERIRQRTHFV
61ISTYREQLQR ALDFRNSSAT CPFLSETIIP SERKKHIFID ITEVRWPSLD TDGTMIYMES
121GIVKITSLDG HAYLCLPRSQ HEFTVHFLCK VSQKSDSSAV LSETNNKAPK DKLVEKTGKI
181CIRGNLPGQR LKNKENEFHC QIMKSKETLK KMSCVNGTEG REELPSPGTK HTCVYTWVKQ
241CWSVAACPEE WKYPLSLALH FHNKISNMSK IDAHITQSRF LTSDISEERG KVVSVLPRAL
301SLSCPVPHLH RWNFCDSLLQ RQSDEYSYPE LVKMVWYKGV TYRLTHQNMN SIEIYSGDGS
361VFKSEGAYFG NYFTYYSIQE GSGKREEKTY SVNNLPPDRP GSPFTVGSLI KQATRILQHC
421VKMRLSLSHN YRICCWKMVP GINDSNILPL VLKESLIPSV GRFLAYSDDK VHAIFLDGIT
481LTLNWNFSSP IEKRQVNQGL NLGWCKLTFP DGQEQLIQIE HPEPYERYVT TVTSWCRRLT
541QTSPREMPTH SSSSVLQENW SVASELEKIQ KFNLLLENSG ILNQISNKKN EQQSFDHYKP
601GSSETLLGEV NENRVSIALK KTSEILHDID CLLSNSKK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

638 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.