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C5orf22 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C5orf22 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111787-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C5orf22 (NM_018356), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C5orf22 | UPF0489 protein C5orf22
Origin Species
Human
NCBI GenBank
NM_018356
SwissProt
Q49AR2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
49.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSDSAGGRAG LRRYPKLPVW VVEDHQEVLP FIYRAIGSKH LPASNVSFLH FDSHPDLLIP
61VNMPADTVFD KETLFGELSI ENWIMPAVYA GHFSHVIWFH PTWAQQIREG RHHFLVGKDT
121STTTIRVTST DHYFLSDGLY VPEDQLENQK PLQLDVIMVK PYKLCNNQEE NDAVSSAKKP
181KLALEDSENT ASTNCDSSSE GLEKDTATQR SDQTCLEPSC SCSSENQECQ TAASTGEILE
241ILKKGKAFVL DIDLDFFSVK NPFKEMFTQE EYKILQELYQ FKKPGTNLTE EDLVDIVDTR
301IHQLEDLEAT FADLCDGDDE ETVQRWASNP GMESLVPLVQ SLKKRMEVPD YEMVHQAGLT
361CDYSELPHHI STEQEIECLI QSVHYLLKNL PNPTLVTIAR SSLDDYCPSD QVDTIQEKVL
421NMLRALYGNL DLQVYAAESP PS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

442 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.