Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
C5AR2 / GPR77 / C5L2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101576-100
Catalog Number: LS-G101576
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C5L2(C5AR2) (NM_018485), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- G-protein coupled receptor 77 | GPF77 | GPR77 | C5L2 | G protein-coupled receptor 77
- Origin Species
- Human
- NCBI GenBank
- NM_018485
- SwissProt
- Q9P296
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 35.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGNDSVSYEY GDYSDLSDRP VDCLDGACLA IDPLRVAPLP LYAAIFLVGV PGNAMVAWVA61GKVARRRVGA TWLLHLAVAD LLCCLSLPIL AVPIARGGHW PYGAVGCRAL PSIILLTMYA121SVLLLAALST DLCFLALGPA WWSTVQRACG VQVACGAAWT LALLLTVPSA IYRRLHQEHF181PARLQCVVDY GGSSSTENAV TAIRFLFGFL GPLVAVVSCH SALLCWAARR CRPLGTAIVV241GFFVCWAPYH LLGLVLTVAA PNSALLARAL RAEPLIVGLA LAHSCLNPML FLYFGRAQLR301RSLPAACHWA LRESQGQDES VDSKKSTSHD LVSEMEVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
337 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.