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C3orf30 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C3orf30 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G106176-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C3orf30 (NM_152539), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C3orf30
Origin Species
Human
NCBI GenBank
NM_152539
SwissProt
Q96M34

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
60 kda
Protein Type
Lysate
Amino Acid Sequence
1MEEPPQEALA EPLKHESPAA PSSAGHTKGQ EEDDQKNQAE RKADNHTAHR IADQTALRVP
61SQAESSIFSQ ATNGVAEQNG HSTPGQAGRR ASNPADVSDL RADDQVNQTP SEQTKGKASS
121QANNVQHEQS DGQVSGLTEE RTAEQTERRL PTQAERRTSG QIDGRLAMPS DQRGSRQTDH
181RMAGQSERRA SEQMDRRMSG EAERRTSEQI THRLSKLSER RPSVQIDSGS SVPSDQSPSV
241QIDSGSSVPS DQRPSVQIDR RMSGKVRRRS SEKTDYRLAG LADPGTSEQT DLRLYGLVDH
301KTSVKTHHQV YGQATELAEH QAIDQAHSNA DQPPVDNAHY TESDQTDHLA DRQANHKDQL
361SYYETRGQSE DRIFPQLGNS KEDKEADYRV QPCKFEDSQV DLNSKPSVEM ETQNATTIPP
421YNPVDARFTS NFQAKDQALF PRLPSILSKL NYTSSQEKTQ AIVTKSDEFS EIDQGKGYHI
481RNQTYRRFPS IVYEDPYQVS LQYMEKHHIL QIFQQITENL VYEKPEDPLN FMLCQV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

536 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.