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C3orf20 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C3orf20 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all C3orf20 antibodies →
$504.00each
Catalog Number: LS-G109025-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C3orf20 (NM_032137), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_032137

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
101.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSYIKSNLEL YQQYTAMAPK LLARISKLLM ICQNAGISVP KGIRNIFEFT WEELISDPSV
61PTPSDILGLE VSFGAPLVVL MEPTFVQVPT LKKPLPPPPP APPRPVLLAT TGAAKRSTLS
121PTMARQVRTH QETLNRFQQQ SIHLLTELLR LKMKAMVESM SVGANPLDIT RRFVEASQLL
181HLNAKEMAFN CLISTAGRSG YSSGQLWKES LANMSAIGVN SPYQLIYHSS TACLSFSLSA
241GKEAKKKIGK SRTTEDVSMP PLHRGVGTPA NSLEFSDPCP EAREKLQELC RHIEAERATW
301KGRNISYPMI LRNYKAKMPS HLMLARKGDS QTPGLHYPPT AGAQTLSPTS HPSSANHHFS
361QHCQEGKAPK KAFKFHYTFY DGSSFVYYPS GNVAVCQIPT CCRGRTITCL FNDIPGFSLL
421ALFNTEGQGC VHYNLKTSCP YVLILDEEGG TTNDQQGYVV HKWSWTSRTE TLLSLEYKVN
481EEMKLKVLGQ DSITVTFTSL NETVTLTVSA NNCPHGMAYD KRLNRRISNM DDKVYKMSRA
541LAEIKKRFQK TVTQFINSIL LAAGLFTIEY PTKKEEEEFV RFKMRSRTHP ERLPKLSLYS
601GESLLRSQSG HLESSIAETL KDEPESAPVS PVRKTTKIHT KAKVTSRGKA REGRSPTRWA
661ALPSDCPLVL RKLMLKEDTR AGCKCLVKAP LVSDVELERF LLAPRDPSQV LVFGIISSQN
721YTSTGQLQWL LNTLYNHQQR GRGSPCIQCR YDSYRLLQYD LDSPLQEDPP LMVKKNSVVQ
781GMILMFAGGK LIFGGRVLNG YGLSKQNLLK QIFRSQQDYK MGYFLPDDYK FSVPNSVLSL
841EDSESVKKAE SEDIQGSSSS LALEDYVEKE LSLEAEKTRE PEVELHPLSR DSKITSWKKQ
901ASKK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

904 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.