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C2orf78 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C2orf78 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G119347-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C2orf78 (NM_001080474), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001080474

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
100 kDa
Protein Type
Lysate
Amino Acid Sequence
1MHWLASATQT SASIVSSSLL SAVDVSSSLT MSEYFQNTSL PGTANSRQFS LPVVSNAAFL
61TGSISNFSRA SAPAISSAWL QPSASGTSFQ PLMGSAYLYQ HSSTTMLSGV TGQSHICTSA
121ASYPGVFEWD STASTVKKSS SLRDFTVTVI DQNTAVSSMS MTAQYYKTSD TNTMVPLYPS
181LSASLVQGTL TQIPNQQGHN LSLPCQIGSQ VYYYNQGTLG PQLSCLQSYG SVSYTGYRAS
241AHQPEMVMVL KEVQPTNVLP PVSTSGMYYS VSSQPITETS VQVMETSLGM DTSLGLQSPS
301QTFCLPQTPE FSKSFSSRNT QTLESNPSPE LGDISITPVQ SPTNLLTLSP APSQEKNENE
361NLDEIKTNLS KPLDVHQILI GNQDPPLLPV EIPDIHPLLA CIDPLGQEEQ PGSENANLRN
421KSLSLEDQGI FENGIESSSD LADITTWVED TYLPPIFSSL QDLDQPESPS AKKAKDTSAI
481KVNQVQEKSC VIKGHSDQVR KNKHKASEPI QGAPKAKIQP KNPECLLERE VVVGSATVSN
541SASVNKAKHS SNKPHKAASS RISKTKSHGQ EKTKGNRKNS SKKSEESKQS GKKVKVEEKQ
601TIPNMKRKKN QPELSQKTLK KPRSSLGMHM LESVQVFHAL GKKIDMKTGF SSSRTLGSSS
661NTQNRQPFPA LKPWLDIQHE GKGPEKIQVK AQKLDGSAEK ECTSPSHSEL PPPGKVKLIP
721LPFLTLDQPQ ARHVSRRPNP LASRRPAVAY PARPDSTNSA QSNAVNPSRP APTNTSLTGP
781ATPAQPISAK ATQPSSANPT QPTVPQSAAS RPSAYKTSSC SSLQREPVST AVTSLRSLPK
841PQNQFLIQDF SLQPRPWRKP TVPEPVMSTP ITEEQRPERE AMKRKAQQER ENAAKYTSLG
901KVQFFIERER DMEIAEYYGY TI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

922 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.