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C2orf71 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C2orf71 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all C2orf71 antibodies →
$725.00each
Catalog Number: LS-G120807-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C2orf71 (NM_001029883), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C2orf71
Origin Species
Human
SwissProt
A6NGG8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
139.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGCTPSHSDL VNSVAKSGIQ FLKKPKAIRP GCQGGSERGS IPLLVKNSTC YDAGEGLAEE
61QPSPRRNQTT AKGLCQLMGD PASGKRKDME GLIPGTKTSS SQLNKSQSHM AKDIPFKTQG
121SHGSQGADFS GDESEESSTQ DTSKWKRTAK CHTSSTQSHC YQTIHPAHEP EGKVDFPEPL
181VKAHQQAYTY LHSSLSKYEA ILCIIHQATQ TRELLQPMVS FLLLCFEEIS QLLGEISKDG
241EVLLQEVRED LAWPLKKREP QEQPNLLQQL LQYTVSKLQV LNGTVASLTG SFLEGSSSYL
301HSTATHLENK LSTKRNVDER LLRALRQLES LASGCGDPGV QGLPLCSEDS GIGADNESVQ
361SVDKLGKQTS WDLAPEPEEW KSVTSPHTEA RQSGHTWQQS PFCLGSGRPQ DCLLSGAPMA
421KVQPRAQDEA RSPCLSSTSP ENITSPPLKL GTSTPCDSFG IGVSVEPHLS KTSRPMDASS
481LSDSEDSSPE EEEEDKMSSM SLCAWQEKTP HSRPQSSPAD RESPFQARTR RLRSLQAQEM
541ILKMKESISE RIKFVPVPCG HQDWSEEEEG RTVVPPRPST VSGSRRAPER QTRSQSESCL
601QSHVEDPTFQ ELRRVQRDLS QKLEAFYALG AKGQGQSQEQ ILQPRAAAVW PNGTCRVSPS
661NTTSRLKASL TKNFSILPSQ DKSILQKCNP HPEDEQGKAG KLPNAIPSGE VSEAAKATDW
721NVRGCPTRTS VKKLIETFSP TESLRMLGDS KDAGASPCLR NCIMPPRFPK YTGLAPLYPK
781PQISPASGRE SLKMGIGWKP LAPIFPPLPK AEAAKSEELS CEMEGNLEHL PPPPMEVLMD
841KSFASLESPE SSKSTENSPK ETQEPGPGEA GPTRRTWASP KLRASVSPLD LLPSKSTASL
901TKPHSTGPGS GRSSCQPRKP ALDLSSPPAT SQSPEVKGGT WSQAEKATSL YRQPRKAIAW
961HHSGPPSGQN RTSESSLARP RQSRERSPPV GRKASPTRTH WVPQADKRRR SLPSSYRPAQ
1021PSPSAVQTPP SPPVSPRVLS PPTTKRRTSP PHQPKLPNPP PESAPAQCKV PSPPTQHPEA
1081SPPFSIPSPS PPMSPSQEHK ETRDSEDSQA VIAKVSGNTH SIFCPATSSL FEAKPPLSTA
1141HPLTPPSLPP EAGGPLGNPA ECWKNSSGPW LRADSQRRAA LCALNPLPFL RRTASDRQPG
1201GRPQPPTLDP TSTSYESQLG QNSSSEESPK KDTEPGSSPC SPELQGGTRR ASPPEFCVLG
1261HGLQPEPRTG HIQDKSQPEA QPQQEEVS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1288 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.