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C2orf42 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C2orf42 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all C2orf42 antibodies →
$504.00each
Catalog Number: LS-G112175-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C2orf42 (NM_017880), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C2orf42
Origin Species
Human
NCBI GenBank
NM_017880
SwissProt
Q9NWW7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEPNSLRTKV PAFLSDLGKA TLRGIRKCPR CGTYNGTRGL SCKNKTCGTI FRYGARKQPS
61VEAVKIITGS DLQVYSVRQR DRGPDYRCFV ELGVSETTIQ TVDGTIITQL SSGRCYVPSC
121LKAATQGVVE NQCQHIKLAV NCQAEATPLT LKSSVLNAMQ ASPETKQTIW QLATEPTGPL
181VQRITKNILV VKCKASQKHS LGYLHTSFVQ KVSGKSLPER RFFCSCQTLK SHKSNASKDE
241TAQRCIHFFA CICAFASDET LAQEFSDFLN FDSSGLKEII VPQLGCHSES TVSACESTAS
301KSKKRRKDEV SGAQMNSSLL PQDAVSSNLR KSGLKKPVVA SSLKRQACGQ LLDEAQVTLS
361FQDWLASVTE RIHQTMHYQF DGKPEPLVFH IPQSFFDALQ QRISIGSAKK RLPNSTTAFV
421RKDALPLGTF SKYTWHITNI LQVKQILDTP EMPLEITRSF IQNRDGTYEL FKCPKVEVES
481IAETYGRIEK QPVLRPLELK TFLKVGNTSP DQKEPTPFII EWIPDILPQS KIGELRIKFE
541YGHHRNGHVA EYQDQRPPLD QPLELAPLTT ITFP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

574 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.