Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
C2GNT3 / GCNT4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G112513-100
Catalog Number: LS-G112513
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C2GNT3(GCNT4) (NM_016591), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Core2-GlcNAc-transferase 3 | Core 2-branching enzyme 3 | GCNT4 | C2GNT3
- Origin Species
- Human
- NCBI GenBank
- NM_016591
- SwissProt
- Q9P109
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKIFKCYFKH TLQQKVFILF LTLWLLSLLK LLNVRRLFPQ KDIYLVEYSL STSPFVRNRY61THVKDEVRYE VNCSGIYEQE PLEIGKSLEI RRRDIIDLED DDVVAMTSDC DIYQTLRGYA121QKLVSKEEKS FPIAYSLVVH KDAIMVERLI HAIYNQHNIY CIHYDRKAPD TFKVAMNNLA181KCFSNIFIAS KLEAVEYAHI SRLQADLNCL SDLLKSSIQW KYVINLCGQD FPLKSNFELV241SELKKLNGAN MLETVKPPNS KLERFTYHHE LRRVPYEYVK LPIRTNISKE APPHNIQIFV301GSAYFVLSQA FVKYIFNNSI VQDFFAWSKD TYSPDEHFWA TLIRVPGIPG EISRSAQDVS361DLQSKTRLVK WNYYEGFFYP SCTGSHLRSV CIYGAAELRW LIKDGHWFAN KFDSKVDPIL421IKCLAEKLEE QQRDWITLPS EKLFMDRNLT TTSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
453 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.