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C2CD2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C2CD2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G113184-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C2CD2 (NM_015500), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C21orf258 | C21orf25 | C2CD2 | C2 domain-containing protein 2 | DKFZP586F0422 | TMEM24L | TMEM24 related geme
Origin Species
Human
SwissProt
Q9Y426

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAMARLGSWL GEAQWLALVS LFVAALATVG LYLAQWALAR ARPQPQRRAV EPGEGPRPGS
61DALLSWILTL GSWRSQWQAA WVTALNEEAE RKGGPPFLSF EEDPRQQALE LVVQEVSSVL
121RSAEEKVVVC HVVGQAIQFL VSETPALGAG CRLYDMRLSP FHLQLEFHMK EKREDLQISW
181SFISVPEMAV NIQPKALGED QVAETSAMSD VLKDILKHLA GSASPSVVLI TKPTTVKEAQ
241NLQCAASTAQ ESCPPKPPRA HELKLLVRNI HVLLLSEPGA SGHINAVCVV QLNDPVQRFS
301STLTKNTPDL MWEEEFTFEL NAKSKELHLQ ISEAGRSSEG LLATATVPLD LFKKQPSGPQ
361SFTLTSGSAC GSSVLGSVTA EFSYMEPGEL KSWPIPPPVP AAKIEKDRTV MPCGTVVTTV
421TAVKTKPRVD VGRASPLSSD SPVKTPIKVK VIEKDISVQA IACRSAPVSK TLSSSDTELL
481VLNGSDPVAE VAIRQLSESS KLKLKSPRKK STIIISGISK TSLSQDHDAA LMQGYTASVD
541STHQEDAPSH PERAAASAPP EEAESAQASL APKPQEDELD SWDLEKEPQA AAWSSQVLLD
601PDGDELSESS MSVLEPGTAK KHKGGILRKG AKLFFRRRHQ QKDPGMSQSH NDLVFLEQPE
661GSRRKGITLT RILNKKLLSR HRNKNTMNGA PVEPCT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

696 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.