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C20orf194 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C20orf194 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all C20orf194 antibodies →
$725.00each
Catalog Number: LS-G121677-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C20orf194 (NM_001009984), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C20orf194
Origin Species
Human
NCBI GenBank
NM_001009984
SwissProt
Q5TEA3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
132.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDVYPPRRQG LPRARSPGGS SRGSPSVSCS RLRQVQSILT QSSKSRPDGI LCILGIDSRY
61NEGCRELANY LLFGLYNQNT SDFEKTGFSE EVLDDVIILI KSDSVHLYCN PVNFRYLLPY
121VAHWRNLHFH CMTENEYEDE EAAEEFKITS FVDMVRDCSR IGIPYSSQGH LQIFDMFVVE
181KWPIVQAFAL EGIGGDGFFT MKYELQDVSL NLWNVYSKMD PMSLESLLSD DLVAFEHQWT
241SFFANFDTEI PFLLELSESQ AGEPFRSYFS HGMISSHITE NSPNRQPFVL FGNHSTRENL
301NAGNFNFPSE GHLVRSTGPG GSFAKHMVAQ CVSPKGPLAC SRTYFFGATH VPYLGGDSKL
361PKKTEQIRLL SQIYAAVIEA VLAGIACYAK TSSLTKAKEV AEQTLGSGLD SFELIPFKAA
421LRSKMTFHIH AVNNQGRIVP LDSEDSLSFV KTACMAVYDI PDLLGGNGCL GSVVFSESFL
481TSQILVKEKD GTVTTETSSV VLTAAVPRFC SWLVEDNEVK LSEKTQQAVR GDESFLGTYL
541TGGEGAYLYS SNLQSWPEEG NVHFFSSGLL FSHCRHRSII ISKDHMNSIS FYDGDSTSTV
601AALLIDFKSS LLPHLPVHFH GSSNFLMIAL FPKSKIYQAF YSEVFSLWKQ QDNSGISLKV
661IQEDGLSVEQ KRLHSSAQKL FSALSQPAGE KRSSLKLLSA KLPELDWFLQ HFAISSISQE
721PVMRTHLPVL LQQAEINTTH RIESDKVIIS IVTGLPGCHA SELCAFLVTL HKECGRWMVY
781RQIMDSSECF HAAHFQRYLS SALEAQQNRS ARQSAYIRKK TRLLVVLQGY TDVIDVVQAL
841QTHPDSNVKA SFTIGAITAC VEPMSCYMEH RFLFPKCLDQ CSQGLVSNVV FTSHTTEQRH
901PLLVQLQSLI RAANPAAAFI LAENGIVTRN EDIELILSEN SFSSPEMLRS RYLMYPGWYE
961GKLNAGSVYP LMVQICVWFG RPLEKTRFVA KCKAIQSSIK PSPFSGNIYH ILGKVKFSDS
1021ERTMEVCYNT LANSLSIMPV LEGPTPPPDS KSVSQDSSGQ QECYLVFIGC SLKEDSIKDW
1081LRQSAKQKPQ RKALKTRGML TQQEIRSIHV KRHLEPLPAG YFYNGTQFVN FFGDKTDFHP
1141LMDQFMNDYV EEANREIEKY NQELEQQEYH DLFELKP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1177 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.