Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
C1QTNF4 / CTRP4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G109138-100
Catalog Number: LS-G109138
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CTRP4(C1QTNF4) (NM_031909), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C1QTNF4 | ZACRP4 | CTRP4
- Origin Species
- Human
- NCBI GenBank
- NM_031909
- SwissProt
- Q9BXJ3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 35.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MLPLLLGLLG PAACWALGPT PGPGSSELRS AFSAARTTPL EGTSEMAVTF DKVYVNIGGD61FDVATGQFRC RVPGAYFFSF TAGKAPHKSL SVMLVRNRDE VQALAFDEQR RPGARRAASQ121SAMLQLDYGD TVWLRLHGAP QYALGAPGAT FSGYLVYADA DADAPARGPP APPEPRSAFS181AARTRSLVGS DAGPGPRHQP LAFDTEFVNI GGDFDAAAGV FRCRLPGAYF FSFTLGKLPR241KTLSVKLMKN RDEVQAMIYD DGASRRREMQ SQSVMLALRR GDAVWLLSHD HDGYGAYSNH301GKYITFSGFL VYPDLAPAAP PGLGASELLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
329 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.