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C1orf38 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C1orf38 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all THEMIS2 antibodies →
$504.00each
Catalog Number: LS-G124150-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C1orf38(THEMIS2) (NM_001105556), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Basement membrane-induced | C1orf38 | ICB-1 | ICB1 | THEMIS2 | Protein THEMIS2 | Protein ICB-1
Origin Species
Human
NCBI GenBank
NM_004848
SwissProt
Q5TEJ8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
71.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEPVPLQDFV RALDPASLPR VLRVCSGVYF EGSIYEISGN ECCLSTGDLI KVTQVRLQKV
61VCENPKTSQT MELAPNFQGY FTPLNTPQSY ETLEELVSAT TQSSKQLPTC FMSTHRIVTE
121GRVVTEDQLL MLEAVVMHLG IRSARCVLGM EGQQVILHLP LSQKGPFWTW EPSAPRTLLQ
181VLQDPALKDL VLTCPTLPWH SLILRPQYEI QAIMHMRRTI VKIPSTLEVD VEDVTASSRH
241VHFIKPLLLS EVLAWEGPFP LSMEILEVPE GRPIFLSPWV GSLQKGQRLC VYGLASPPWR
301VLASSKGRKV PRHFLVSGGY QGKLRRRPRE FPTAYDLLGA FQPGRPLRVV ATKDCEGERE
361ENPEFTSLAV GDRLEVLGPG QAHGAQGSDV DVLVCQRLSD QAGEDEEEEC KEEAESPERV
421LLPFHFPGSF VEEMSDSRRY SLADLTAQFS LPCEVKVVAK DTSHPTDPLT SFLGLRLEEK
481ITEPFLVVSL DSEPGMCFEI PPRWLDLTVV KAKGQPDLPE GSLPIATVEE LTDTFYYRLR
541KLPACEIQAP PPRPPKNQGL SKQRRHSSEG GVKSSQVLGL QQHARLPKPK AKTLPEFIKD
601GSSTYSKIPA HRKGHRPAKP QRQDLDDDEH DYEEILEQFQ KTI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

643 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.