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C1orf26 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C1orf26 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G112295-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SWT1 (NM_017673), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HsSwt1 | Transcriptional protein SWT1 | C1orf26 | SWT1
Origin Species
Human
NCBI GenBank
NM_017673
SwissProt
Q5T5J6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
103 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSSKESCGKK ETSQRKDTTT SSPNFGEKDK KERKTPASST SSSSIRSVSS EKRKLKSDHT
61DVLYYNIKRR QGLKRLSVEI DTLRRRPKIG SSSQRPIKLK EASYSNDNQI ILQSPSSNGT
121KKDIHKCVDF KPKDIKLTNA GSKLDHGVKS LSSPKIASDV KPKAEGQASE NKWSHLLVQR
181EKMKELKKGR NSKFRDNSEK CVLEKWKRNQ FSQDYNSNKI IKEPLGSRRQ KISFKIPIKS
241RDTLQKLVEE NVFNIDSNNS KTKQEEREYL ESSQVSLNVT RQKTEHLLSD FTYKRTVHEW
301KRKHHYDHQE SNDSHSRENL TQSFEAPCCS VSSESIQDAD QEMQIVEELH AARVGKSVDL
361PGELMSMEID LEDDVHSSSA NNTSDRKLLI VIDTNILMNH LKFVRILKTT EVPGFDKLVL
421IIPWVVMQEL DRMKEGKLLK RAQHKAIPAV HFINDSLKNQ DRKLWGQSIQ LASQKHYGLS
481DENNDDRVLK CCLQHQELFP CSFVILCTDD RNLRNKGLIS GVKSLSKEEL SAELLRLSLN
541TDVCHQPCIP KQQLKAETTP LKESYKEEST SSGLSILLES IVSDLEKSLG TGLSSILETE
601MKIAFGNLWM EILYLKPPWT LLHLLQCFKK HWLAVFGLVM EKNLLLTIES LYKNLRKANK
661AVDFTTVKFL LQDSRSLLHA FSTRSNYDGI LPQTFAQVNN LLQTFAEVKT KLKPNSSENT
721VTKKQEGTSL KNSHNQEITV FSSSHLPQPS RHQEIWSILE SVWITIYQNS TDVFQRLGSN
781SALTTSNIAS FEEAFICLQK LMAAVRDILE GIQRILAPNS DYQDVETLYN FLIKYEVNKN
841VKFTAQEIYD CVSQTEYREK LTIGCRQLVE MEYTMQQCNA SVYMEAKNRG WCEDMLNYRI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

900 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.