Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
C1orf2 / FAM189B Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G103758-100
Catalog Number: LS-G103758
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C1orf2(FAM189B) (NM_198264), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- COTE1 | Protein FAM189B | Protein COTE1 | C1orf2 | FAM189B
- Origin Species
- Human
- NCBI GenBank
- NM_006589
- SwissProt
- P81408
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MMPSPSDSSR SLTSRPSTRG LTHLRLHRPW LQALLTLGLV QVLLGILVVT FSMVASSVTT61TESIKRSCPS WAGFSNLLFS VCGLTICAAI ICTLSAIVCC IQIFSLDLVH TLAPERSVSG121PLGPLGCTSP PPAPLLHTML DLEEFVPPVP PPPYYPPEYT CSSETDAQSI TYNGSMDSPV181PLYPTDCPPS YEAVMGLRGD SQATLFDPQL HDGSCICERV ASIVDVSMDS GSLVLSAIGD241LPGGSSPSED SCLLELQGSV RSVDYVLFRS IQRSRAGYCL SLDCGLRGPF EESPLPRRPP301RAARSYSCSA PEAPPPLGAP TAARSCHRLE GWPPWVGPCF PELRRRVPRG GGRPAAAPPT361RAPTRRFSDS SGSLTPPGHR PPHPASPPPL LLPRSHSDPG ITTSSDTADF RDLYTKVLEE421EAASVSSADT GLCSEACLFR LARCPSPKLL RARSAEKRRP VPTFQKVPLP SGPAPAHSLG481DLKGSWPGRG LVTRFLQISR KAPDPSGTGA HGHKQVPRSL WGRPGRESLH LRSCGDLSSS541SSLRRLLSGR RLERGTRPHS LSLNGGSRET GLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
572 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.