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C1orf168 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C1orf168 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all C1orf168 antibodies →
$725.00each
Catalog Number: LS-G122562-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C1orf168 (NM_001004303), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C1orf168 | RP4-758N20.2
Origin Species
Human
NCBI GenBank
NM_001004303
SwissProt
Q5VWT5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
81.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEGEGVRNFK ELRAKFQNLD APPLPGPIKF PAGVSPKGDI GGTQSTQILA NGKPLSSNHK
61QRTPYCSSSE SQPLQPQKIK LAQKSEIPKC SNSPGPLGKS TVCSATSSQK ASLLLEVTQS
121NVEIITKEKV MVANSFRNKL WNWEKVSSQK SEMSSALLLA NYGSKAIHLE GQKGMGLTPE
181EPRKKLETKG AQTLPSQKHV VAPKILHNVS EDPSFVISQH IRKSWENPPP ERSPASSPCQ
241PIYECELASQ APEKQPDVRH HHLPKTKPLP SIDSLGPPPP KPSRPPIVNL QAFQRQPAAV
301PKTQGEVTVE EGSLSPERLF NAEFEEPHNY EATISYLRHS GNSINLCTAK EIADPTYEVG
361IEELQKPGKN FPYPEPSAKH EDKKMKEKQP CELKPKNTEK EPYSNHVFKV DACEGTPEKI
421QMTNVHTGRR NMLAGKQEAM IDIIQTNPCP EGPKLARHSQ GHCGHLEVLE STKETPDLGV
481SKTSSISEEI YDDVEYSRKE VPKLNYSSSL ASSSEENREL YEDVYKTKNN YPKIDLDGKE
541ALKRLQQFFK KEKDRFKIKK TKSKENLSAF SILLPDLELK SQEVIIYDDV DLSEKESKDE
601DKLKMWKPKF LTPKEKKEKN GAEESESFSP RNFFKTKKQN LEKNRMKREE KLFRERFKYD
661KEIIVINTAV ACSNNSRNGI FDLPISPGEE LEVIDTTEQN LVICRNSKGK YGYVLIEHLD
721FKHQSWSP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

728 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.