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C1orf127 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C1orf127 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all C1orf127 antibodies →
$725.00each
Catalog Number: LS-G130251-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C1orf127 (NM_001170754), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C1orf127
Origin Species
Human
NCBI GenBank
NM_001170754
SwissProt
Q8N9H9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
89.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MWGSPALAWA VWLACVQPTV FPWSLSFRSD TDKPSSAAEV LTEASSCWTD TVECFSDYMT
61LWIPRSHVEG LRRWLARTLH LPGTWRSPDH LDSSLAKCGY FLHLASDGDF LFRVQYSACF
121VQKEKANYRL EIRIFQKGVM GLERSDRYIM KCPMLRSRLG QESVHCGPMF IQVSRPLPLW
181RDNRQTPWLL SLRGELVASL EDASLMGLYV DMNATTVTVQ SPRQGLLQRW EVLNTSAELL
241PLWLVSGHHA YSLEAACPPV SFQPESEVLV HIPKQRLGLV KRGSYIEETL SLRFLRVHQS
301NIFMVTENKD FVVVSIPAAG VLQVQRCQEV GGTPGTQAFY RVDLSLEFAE MAAPVLWTVE
361SFFQCVGSGT ESPASTAALR TTPSPPSPGP ETPPAGVPPA ASSQVWAAGP AAQEWLSRDL
421LHRPSDALAK KGLGPFLQTA KPARRGQTSA SILPRVVQAQ RGPQPPPGEA GIPGHPTPPA
481TLPSEPVEGV QASPWRPRPV LPTHPALTLP VSSDASSPSP PAPRPERPES LLVSGPSVTL
541TEGLGTVRPE QDPAKSPGSP LLLRGLSSGD VAAPEPIMGE PGQASEEFQP LARPWRATLA
601AEELVSHRSP GEPQETCSGT EVERPRQTGP GLPREGARGH MDLSSSEPSQ DIEGPGLSIL
661PARDATFSTP SVRQPDPSAW LSSGPELTGM PRVRLAAPLA VLPMEPLPPE PVRPAALLTP
721EASSVGGPDQ ARYLESAPGW PVGQEEWGVA HTSSPPSTQT LSLWAPTGVL LPSLVELEYP
781FQAGRGASLQ QELTEPTLAL SAESHRPPEL QDSVEGLSER PSR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

823 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.