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C1orf116 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C1orf116 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all C1orf116 antibodies →
$504.00each
Catalog Number: LS-G110131-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SARG(C1orf116) (NM_023938), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C1orf116 | SARG
Origin Species
Human
NCBI GenBank
NM_023938
SwissProt
Q9BW04

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPERELWPAG TGSEPVTRVG SCDSMMSSTS TRSGSSDSSY DFLSTEEKEC LLFLEETIGS
61LDTEADSGLS TDESEPATTP RGFRALPITQ PTPRGGPEET ITQQGRTPRT VTESSSSHPP
121EPQGLGLRSG SYSLPRNIHI ARSQNFRKST TQASSHNPGE PGRLAPEPEK EQVSQSSQPR
181QAPASPQEAA LDLDVVLIPP PEAFRDTQPE QCREASLPEG PGQQGHTPQL HTPSSSQERE
241QTPSEAMSQK AKETVSTRYT QPQPPPAGLP QNARAEDAPL SSGEDPNSRL APLTTPKPRK
301LPPNIVLKSS RSSFHSDPQH WLSRHTEAAP GDSGLISCSL QEQRKARKEA LEKLGLPQDQ
361DEPGLHLSKP TSSIRPKETR AQHLSPAPGL AQPAAPAQAS AAIPAAGKAL AQAPAPAPGP
421AQGPLPMKSP APGNVAASKS MPIPIPKAPR ANSALTPPKP ESGLTLQESN TPGLRQMNFK
481SNTLERSGVG LSSYLSTEKD ASPKTSTSLG KGSFLDKISP SVLRNSRPRP ASLGTGKDFA
541GIQVGKLADL EQEQSSKRLS YQGQSRDKLP RPPCVSVKIS PKGVPNEHRR EALKKLGLLK
601ETRTRPLEQK LISEEDLAAN DILDYKDDDD KV

632 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.