Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
C1orf107 / DEF Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113975-100
Catalog Number: LS-G113975
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DIEXF (NM_014388), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C1orf107 | DJ434O14.5 | DIEXF | UTP25 | DEF
- Origin Species
- Human
- NCBI GenBank
- NM_014388
- SwissProt
- Q68CQ4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 86.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGKRGSRSQS QLLNTLTKKQ KKHLRDFGEE HPFYDRVSRK EAKPQICQLS ESSDSSDSES61DSESEPQQVS GYHRLLATLK NVSEEEEEDE EEEEEEDSIV DDAEMNDEDG GSDVSVEEEM121AAESTESPEN VALSADPEGK EDGEEPPGTS QTSPEEFTDA KHESLFSLET NFLEEESGDN181SSLKASQDPF LQHVNKELKE KAIQAVATNP KTTHELKWPI LGQLFFSSKF QKLETFKPPK241DIDLKSLHLQ KPLESTWTKT NSQFLSGPQK SSSPFTPLQK ELFLIMNSYR DLFYPERTAL301KNGEEIRHVY CLHVINHILK ANAQVLGNNS RRRSQKFGVG DDDDFRDQGL TRPKVLIVVP361FREAALRVVQ LFISLLEGDS KKKIIVSNKK RFQGEYGSDP EERPPNLKRP EDYEAVFVGN421IDDHFRIGVA ILQRSIRLYA PFYSSDILIA SPLGLRTIIG GEGEKKRDFD FLSSIELLII481DQADIYLMQN WEHVLHLMNH MNLLPLDSHG VDFSRVRMWS LNNWSKYYRQ TLLFGALQDA541QINSVFNKYC VNMQGQVAVR NVPMTGSISH VLVQLPHVFQ RMEAENLASV IDARFNFFVN601KILPQYRDAV MSHTLIYIPS YFDFVRLRNY FKKEELNFTH ICEYTQKSGV SRARHFFLQG661EKQFLLFTER FHFYKRYTIK GIRNLIFYEL PTYPHFYSEI CNMLRATNRG EEATWTCTVL721YSKYDAQRLA AVVGVERAAQ MLQSNKNVHL FITGEKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
756 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.