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C1orf106 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C1orf106 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all C1orf106 antibodies →
$725.00each
Catalog Number: LS-G111854-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C1orf106 (NM_018265), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C1orf106
Origin Species
Human
NCBI GenBank
NM_018265
SwissProt
Q3KP66

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLQMPKLNEI PPGRAGRREA RGEGRWPGQT GPEAARLEWR AQGQAGGARA PWDSWGSSRL
61PTQPGPGWSR CPPSLLCALS FQKSTMESKD EVSDTDSGII LQSGPDSPVS PMKELTHAVH
121KQQRALEARL EACLEELRRL CLREAELTGT LPAEYPLKPG EKAPKVRRRI GAAYKLDDWA
181LHREDPLSSL ERQLALQLQI TEAARRLCLE ENLSRQARRQ RKHSMLQEEK KLQELQRCLV
241ERRRNSEPPP AAALPLGREL SASDDSSLSD GLLLEEEESQ VPKPPPESPA PPSRPLPPQT
301LEGLQPTGPE AGSPERAPVQ NSPWKETSLD HPYEKPRKSS EPWSESSSPA TTPQDGPSAS
361SLWLLEPASY HVVPIRGVPG QWQGRTSAPA TPEIQGRRGQ SQSLRVDSFR AGPEGRGRSA
421FPRRRPTHYT VTVPDSCFPA TKPPLPHAAC HSCSEDSGSD VSSISHPTSP GSSSPDISFL
481QPLSPPKTHR HRGAWVPAGS RELVAHHPKL LLPPGYFPAG RYVVVAESPL PPGEWELCRA
541APGPAYEEEG TPLRYQRLVP SRSRIVRTPS LKDSPAGRGL SKAAVSEELK WWHERARLRS
601TRPHSLDRQG AFRVRSLPLG REGFGRALGP RAQVPTVCVL RRSPDGAPVQ VFVPEKGEII
661SQV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

663 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.