Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
C18orf8 / MIC1; Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G114293-100
Catalog Number: LS-G114293
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C18orf8 (NM_013326), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C18orf8 | MIC1 | MIC-1 | HsT2591
- Origin Species
- Human
- NCBI GenBank
- NM_013326
- SwissProt
- Q96DM3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 74.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGEEDYYLEL CERPVQFEKA NPVNCVFFDE ANKQVFAVRS GGATGVVVKG PDDRNPISFR61MDDKGEVKCI KFSLENKILA VQRTSKTVDF CNFIPDNSQL EYTQECKTKN ANILGFCWTS121STEIVFITDQ GIEFYQVLPE KRSLKLLKSH NLNVNWYMYC PESAVILLST TVLENVLQPF181HFRAGTMSKL PKFEIELPAA PKSTKPSLSE RDIAMATIYG QLYVLFLRHH SRTSNSTGAE241VVLYHLPREG ACKKMHILKL NRTGKFALNV VDNLVVVHHQ DTETSVIFDI KLRGEFDGSV301TFHHPVLPAR SIQPYQIPIT GPAAVTSQSP VPCKLYSSSW IVFQPDIIIS ASQGYLWNLQ361VKLEPIVNLL PDKGRLMDFL LQRKECKMVI LSVCSQMLSE SDRASLPVIA TVFDKLNHEY421KKYLDAEQSY AMAVEAGQSR SSPLLKRPVR TQAVLDQSDV YTHVLSAFVE KKEMPHKFVI481AVLMEYIRSL NQFQIAVQHY LHELVIKTLV QHNLFYMLHQ FLQYHVLSDS KPLACLLLSL541ESFYPPAHQL SLDMLKRLST ANDEIVEVLL SKHQVLAALR FIRGIGGHDN ISARKFLDAA601KQTEDNMLFY TIFRFFEQRN QRLRGSPNFT PGEHCEEHVA FFKQIFGDQA LMRPTTFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
657 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.