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C18orf54 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

C18orf54 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G105182-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C18orf54 (NM_173529), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C18orf54 | Hypothetical protein V1 | Hypothetical protein V4 | LAS2 | Hypothetical protein V3 | Hypothetical protein V5
Origin Species
Human
SwissProt
Q8IYD9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
41.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MAKSKTKHRL CSQESSVSAL LASCTLSGSN SSNSDGSFHY KDKLYRSASQ ALQAYIDDFD
61LGQIYPGAST GKINIDEDFT NMSQFCNYIY KPNNAFENLD HKKHSNFISC RRHTVNDIDS
121MSLTTDDLLR LPADGSFSYT YVGPSHRTSK KNKKCRGRLG SLDIEKNPHF QGPYTSMGKD
181NFVTPVIRSN INGKQCGDKI ELLILKAKRN LEQCTEELPK SMKKDDSPCS LDKLEADRSW
241ENIPVTFKSP VPVNSDDSPQ QTSRAKSAKG VLEDFLNNDN QSCTLSGGKH HGPVEALKQM
301LFNLQAVQER FNQNKTTDPK EEIKQVSEDD FSKLQLKESM IPITRSLQKA LHHLSRLRDL
361VDDTNGERSP KM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

372 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.