Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
C18orf25 / ARKL1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G121910-100
Catalog Number: LS-G121910
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C18orf25 (NM_001008239), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ARKadia-like 1 | C18orf25 | ARKL1
- Origin Species
- Human
- SwissProt
- Q96B23
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 36.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MKMEEAVGKV EELIESEAPP KASEQETAKE EDGSVELESQ VQKDGVADST VISSMPCLLM61ELRRDSSESQ LASTESDKPT TGRVYESDSS NHCMLSPSSS GHLADSDTLS SAEENEPSQA121ETAVEGDPSG VSGATVGRKS RRSRSESETS TMAAKKNRQS SDKQNGRVAK VKGHRSQKHK181ERIRLLRQKR EAAARKKYNL LQDSSTSDSD LTCDSSTSSS DDDEEVSGSS KTITAEIPGH241LDPGFLASDK TSAGNAPLNE EINIASSDSE VEIVGVQEHA RCVHPRGGVI QSVSSWKHGS301GTQYVSTRQT QSWTAVTPQQ TWASPAEVVD LTLDEDSRRK YLLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
343 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.